Effects of cloning and root-tip size on observations of fungal ITS sequences from Picea glauca roots

Daniel Lindner; Mark Banik

Treesearch

Effects of cloning and root-tip size on observations of fungal ITS sequences from Picea glauca roots

Informally Refereed

Authors:

Daniel Lindner, Mark Banik

Year:

2009

Type:

Scientific Journal

Station:

Northern Research Station

Source:

Mycologia. 101(1): 157?165.

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Abstract

To better understand the effects of cloning on observations of fungal ITS sequences from Picea glauca (white spruce) roots two techniques were compared: (i) direct sequencing of fungal ITS regions from individual root tips without cloning and (ii) cloning and sequencing of fungal ITS regions from individual root tips. Effect of root tip size was investigated by selecting 20 small root tips (SRT, 1.0-2.0 mm long) and 20 large root tips (LRT, 5.0- 6.0 mm long). DNA was isolated from each tip and PCR-amplified with fungal-specific primers.

Keywords

ectomycorrhizal fungi, endophyte, environmental samples, mycorrhiza, species identification

Citation

Lindner, Daniel L.; Banik, Mark T. 2009. Effects of cloning and root-tip size on observations of fungal ITS sequences from Picea glauca roots. Mycologia. 101(1): 157-165.