Enzyme immunoassays for detection of gypsy moth nuclear polyhedrosis virus

Michael Ma

Treesearch

Enzyme immunoassays for detection of gypsy moth nuclear polyhedrosis virus

Informally Refereed

Authors:

Michael Ma

Year:

1985

Type:

General Technical Report

Station:

Northern Research Station

Source:

In: Grimble, David G.; Lewis, Franklin B., coords. Proceedings, Symposium: Microbial control of spruce budworms and gypsy moths; 1984 April 10-12; Windsor Locks, CT. Gen. Tech. Rep. NE-100. Broomall, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station. 125-131

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Abstract

Enzyme-linked immunosorbent assays (ELISA) were developed for detecting gypsy moth (Lymantria dispar L.) nuclear polyhedrosis virus (NPV). They were used to detect the presence of NPV in hemoplymph samples collected from infected larvae. The incorporation of hybridoma antibodies with these procedures would make them even more specific for gypsy moth NPV detection. A pilot study of evaluating NPV spray with ELISA is also presented here. Potentially, these immunochemical methods have important applications in an IPM approach toward the control of gypsy moth.

Parent Publication

Proceedings, Symposium: Microbial control of spruce budworms and gypsy moths

Citation

Ma, Michael. 1985. Enzyme immunoassays for detection of gypsy moth nuclear polyhedrosis virus. In: Grimble, David G.; Lewis, Franklin B., coords. Proceedings, Symposium: Microbial control of spruce budworms and gypsy moths; 1984 April 10-12; Windsor Locks, CT. Gen. Tech. Rep. NE-100. Broomall, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station. 125-131