Cloning and sequencing of a cellobiohydrolase gene from Trichoderma harzianum FP108

Patrick Guilfoile; Ron Burns; Zu-Yi Gu; Matt Amundson; Fu-Hsian Chang

Treesearch

Cloning and sequencing of a cellobiohydrolase gene from Trichoderma harzianum FP108

Informally Refereed

Authors:

Patrick Guilfoile, Ron Burns, Zu-Yi Gu, Matt Amundson, Fu-Hsian Chang

Year:

1999

Type:

Scientific Journal

Station:

Northern Research Station

Source:

Journal of the Minnesota Academy of Science. 64(1): 18-22. (1999)

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Abstract

A cbbl cellobiohydrolase gene was cloned and sequenced from the fungus Trichoderrna harzianum FP108. The cloning was performed by PCR amplification of T. harzianum genomic DNA, using PCR primers whose sequence was based on the cbbl gene from Tricboderma reesei. The 3' end of the gene was isolated by inverse PCR; attempts to clone regions upstream of the 5' end of the gene were unsuccessful. Sequence comparisons suggest that this gene is closely related to cbbl genes from other Trichoderma species. In particular, all catalytically important amino acids in the protein sequence deduced from the T. harzianum cbbl gene are conserved between species.

Keywords

cloning, cellobiohydrolase, Trichoderma harzianum, DNA, genes

Citation

Guilfoile, Patrick; Burns, Ron; Gu, Zu-Yi; Amundson, Matt; Chang, Fu-Hsian. 1999. Cloning and sequencing of a cellobiohydrolase gene from Trichoderma harzianum FP108. Journal of the Minnesota Academy of Science. 64(1): 18-22. (1999)