Identification of culturable stream water bacteria from urban, agricultural, and forested watersheds using 16S rRNA gene sequencing

Ken Belt; Christina Hohn; Aiah Gbakima; James A. Higgins

Treesearch

Identification of culturable stream water bacteria from urban, agricultural, and forested watersheds using 16S rRNA gene sequencing

Informally Refereed

Authors:

Ken Belt, Christina Hohn, Aiah Gbakima, James A. Higgins

Year:

2007

Type:

Scientific Journal

Station:

Northern Research Station

Source:

Journal of Water and Health. 5(3): 395-406.

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Abstract

Bacteria present in water samples taken on a weekly basis, from June 2004 through June 2005, from three streams, were cultured on Coliscan® Easygel® agar plates. Colonies representative of a variety of colors and morphologies were subjected to amplification and sequencing of a 1000-1100 nt portion of the 16S rRNA gene. A total of 528 colonies were sequenced; these categorized into 26 genera and 78 species. Of 175 dark blue/purple colonies presumed to be E. coli, sequence analysis indicated that 45 (25%) were actually other genera.

Keywords

16S rRNA, bacterial identification, freshwater bacteria, stream flora

Citation

Belt, Kenneth T.; Hohn, Christina; Gbakima, Aiah; Higgins, James A. 2007. Identification of culturable stream water bacteria from urban, agricultural, and forested watersheds using 16S rRNA gene sequencing. Journal of Water and Health. 5(3): 395-406.