Construction of a transfer vector for a clonal isolate of LdNPV

Shivanand Hiremath; Martha Fikes; Audrey Ichida

Treesearch

Construction of a transfer vector for a clonal isolate of LdNPV

Informally Refereed

Authors:

Shivanand Hiremath, Martha Fikes, Audrey Ichida

Year:

1991

Type:

General Technical Report

Station:

Northern Research Station

Source:

IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 53.

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Abstract

Deoxyribonucleic acid from a clonal isolate of LdNPV (CI A2-1), obtained by in vivo cloning procedures, was used to construct genomic libraries in phage (lamda Gem 11) and cosmid (pHC79) vectors. Overlapping clones were selected to generate a restriction enzyme map. The restriction enzyme map, covering about 85% of the CI A2-1 genome, was determined. Efforts are underway to clone and characterize the missing regions.

Parent Publication

Proceedings, U. S. Department of Agriculture interagency gypsy moth research review 1990

Citation

Hiremath, Shivanand T.; Fikes, Martha; Ichida, Audrey. 1991. Construction of a transfer vector for a clonal isolate of LdNPV. IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 53.