Isolation and characterization of juvenile hormone esterase from gypsy moth (Lymantria dispar)

Al Valaitis; Joan Jolliff

Treesearch

Isolation and characterization of juvenile hormone esterase from gypsy moth (Lymantria dispar)

Informally Refereed

Authors:

Al Valaitis, Joan Jolliff

Year:

1991

Type:

General Technical Report

Station:

Northern Research Station

Source:

IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 82.

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Abstract

Insect metamorphosis is under precise hormonal control. During the last larval stadium, the degradation of juvenile hormone by juvenile hormone esterase (JHE) is essential for the initiation of pupation. Therefore, we have targeted this system for disruption with a strategy to produce a recombinant gypsy moth virus which expresses JHE. In order to clone and insert the JHE gene into the virus, purification of the enzyme, amino acid sequence information, and polyclonal antibody were needed.

Parent Publication

Proceedings, U. S. Department of Agriculture interagency gypsy moth research review 1990

Citation

Valaitis, Algimantas P.; Jolliff, Joan. 1991. Isolation and characterization of juvenile hormone esterase from gypsy moth (Lymantria dispar). IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 82.