Use of molecular probes to detect parasites and retrotransposons in gypsy moths

John H. Werren; Thomas O'Dell

Treesearch

Use of molecular probes to detect parasites and retrotransposons in gypsy moths

Informally Refereed

Authors:

John H. Werren, Thomas O'Dell

Year:

1991

Type:

General Technical Report

Station:

Northern Research Station

Source:

IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 84.

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Abstract

Retrotransposon screen: Gypsy moth families containing straggling and nonstraggling individuals were divided into categories of straggling, medium, and nonstraggling individuals, from which DNA was extracted. Four families were tested by southern hybridization and probing with ribosomal sequences designed to detect R1 and R2 retrotransposon insertions. Results showed no differences between stragglers and nonstragglers in the proportion of insertions in their ribosomal genes. The average proportion of insertions was 30%.

Parent Publication

Proceedings, U. S. Department of Agriculture interagency gypsy moth research review 1990

Citation

Werren, John H.; O''Dell, Thomas. 1991. Use of molecular probes to detect parasites and retrotransposons in gypsy moths. IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 84.