Survey and Detection of Endophytic Fungi in Lolium Germ Plasm by Direct Staining and Aphid Assays

Dan D. Wilson; Stephen L. Clement; Walter J. Kaiser

Treesearch

Survey and Detection of Endophytic Fungi in Lolium Germ Plasm by Direct Staining and Aphid Assays

Informally Refereed

Authors:

Dan D. Wilson, Stephen L. Clement, Walter J. Kaiser

Year:

1991

Type:

Scientific Journal

Station:

Southern Research Station

Source:

Plant Disease. 75:169-173, February 1991

Download (PDF 321 KB)

Download (PDF 321 KB): https://research.fs.usda.gov/download/treesearch/1584.pdf

Abstract

Clavicipitaceous anamorphic endophytes were detected in 28 of 85 accessions from five of eight species in a collection of Lolium germ plasm. Comparative descriptions of endophytic mycelium in seeds of L. multiflorum, L. perenne, L. persicum, L. rigidum, and L. temulentum revealed morphological characteristics distinguishing endophytes in each host species. Most endophyte-infected accessions originated from Eurasia, particularly from Middle Eastern countries. Endophyte incidence and infection percentages varied widely with host species and geographic origin. Feeding tests with Diuruphis noxia were useful in detecting endophyte-infected accessions of perennial ryegrass.

Keywords

Acremonium, bioassay, biological control, Clavicipitaceae, Russian wheat aphid

Citation

Wilson, A. Dan; Clement, Stephen L.; Kaiser, Walter J. 1991. Survey and Detection of Endophytic Fungi in Lolium Germ Plasm by Direct Staining and Aphid Assays. Plant Disease. 75:169-173, February 1991