Regeneration of plants from Fraxinus pennsylvanica hypocotyls and cotyledons

Ningxia Du; Paula M. M. Pijut

doi:10.1016/j.scienta.2008.05.014

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Regeneration of plants from Fraxinus pennsylvanica hypocotyls and cotyledons

Informally Refereed

Authors:

Ningxia Du, Paula M. M. Pijut

Year:

2008

Type:

Scientific Journal

Station:

Northern Research Station

DOI:

https://doi.org/10.1016/j.scienta.2008.05.014

Source:

Scientia Horticulturae. 118: 74-79.

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Abstract

An adventitious shoot regeneration and rooting protocol was developed for green ash (Fraxinus pennsylvanica) seedling explants. The best regeneration medium for freshly isolated hypocotyls and cotyledons was Murashige and Skoog (MS) supplemented with 13.3 µM6-benzylaminopurine (BA) plus 4.5 µM thidiazuron (TDZ), and 22.2 µM BA plus 4.5 µM TDZ, respectively. Seventy-six percent of hypocotyls segments and 24% of cotyledon segments produced adventitious shoots, with a mean number of adventitious shoots per explant of 2.7±0.5 and 2.3±1.3, respectively. The effect of invitro-germinated seedling age on adventitious shoot regeneration from hypocotyls andcotyledon explants was also studied.

Keywords

adventitious shoots, green ash, rooting, shoot organogenesis, tissue culture

Citation

Du, Ningxia; Pijut, Paula M. 2008. Regeneration of plants from Fraxinus pennsylvanica hypocotyls and cotyledons. Scientia Horticulturae. 118: 74-79.