In vitro propagation of northern red oak (Quercus rubra L.)

Abstract

In vitro propagation of northern red oak (Quercus rubra) shoots was successful from cotyledonary node explants excised from 8-wk-old in vitro grown seedlings. Initially, four shoots per explant were obtained on Murashige and Skoog (MS) medium supplemented with 4.4 µM 6-benzylaminopurine (BA), 0.45 µM thidiazuron (TDZ), and 500 mg l⁻¹ casein hydrolysate (CH) with a regeneration frequency of 64.7% after 3 wk. Subculturing explants (after harvesting shoots) to fresh treatment medium significantly increased shoot bud regeneration (16.6 buds per explant), but the buds failed to develop into shoots.

Citation

Vengadesan, G.; Pijut, Paula M. 2009. In vitro propagation of northern red oak (Quercus rubra L.). In Vitro Cellular and Developmental Biology-Plant. 45: 474-482.