Agrobacterium-mediated transformation of Fraxinus pennsylvanica hypocotyls and plant regeneration

Ningxia Du; Paula M. M. Pijut

doi:10.1007/s00299-009-0697-z

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Agrobacterium-mediated transformation of Fraxinus pennsylvanica hypocotyls and plant regeneration

Informally Refereed

Authors:

Ningxia Du, Paula M. M. Pijut

Year:

2009

Type:

Scientific Journal

Station:

Northern Research Station

DOI:

https://doi.org/10.1007/s00299-009-0697-z

Source:

Plant Cell Reports. 28: 915-923.

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Abstract

A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and β-glucuronidase (GUS) fusion gene, and an enhanced green fluorescent protein gene. Pre-cultured hypocotyl explants were transformed in the presence of 100 µM acetosyringone using 90 s sonication plus 10 min vacuum-infiltration.

Keywords

genetic engineering, green ash, sonication, transgenic, vacuum-infiltration

Citation

Du, Ningxia; Pijut, Paula M. 2009. Agrobacterium-mediated transformation of Fraxinus pennsylvanica hypocotyls and plant regeneration. Plant Cell Reports. 28: 915-923.