Peroral bioassay of nucleopolyhedrosis viruses in larvae of western spruce budworm.

Mauro E. Martignoni; Paul J. Iwai

doi:10.2737/PNW-RP-285

Treesearch

Peroral bioassay of nucleopolyhedrosis viruses in larvae of western spruce budworm.

Informally Refereed

Authors:

Mauro E. Martignoni, Paul J. Iwai

Year:

1981

Type:

Research Paper

Station:

Pacific Northwest Research Station

DOI:

https://doi.org/10.2737/PNW-RP-285

Source:

Res. Pap. PNW-RP-285. Portland, OR: U.S. Department of Agriculture, Forest Service. 20 p.

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Abstract

The relative virulence of entomopathogenic viruses and the potency of virus preparations for control of destructive insects can be estimated reliably only by means of biological assay in the target species. A simple, yet sensitive peroral bioassay procedure is described for preparations of nucleopolyhedrosis viruses pathogenic for the western spruce budworm, Choristoneura occidentalis Freeman. This procedure has been used to compare the activity of Baculovirus isolates from several conifer-feeding species of Choristoneura.

Keywords

Bioassay, Baculovirus, nucleopolyhedrosis virus, virus (insect), diseases (insect), quality control (virus preparations), biological control (forest pests), western spruce budworm, Choristoneura occidentalis

Citation

Martignoni, Mauro E.; Iwai, Paul J. 1981. Peroral bioassay of nucleopolyhedrosis viruses in larvae of western spruce budworm. Res. Pap. PNW-RP-285. Portland, OR: U.S. Department of Agriculture, Forest Service, Pacific Northwest Forest and Range Experiment Station. 20 p.