Xylitol production by a Pichia stipitis D-xylulokinase mutant

Yong-Su Jin; Jose Cruz; Thomas W. Jeffries

doi:10.1007/s00253-004-1854-5

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Xylitol production by a Pichia stipitis D-xylulokinase mutant

Informally Refereed

Authors:

Yong-Su Jin, Jose Cruz, Thomas W. Jeffries

Year:

2005

Type:

Miscellaneous Publication

Station:

Forest Products Laboratory

DOI:

https://doi.org/10.1007/s00253-004-1854-5

Source:

Applied microbiology and biotechnology. Vol. 68, no. 1 (2005): pages 42-45.

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Abstract

Xylitol production by Pichia stipitis FPL-YS30, a xyl3-Ä1 mutant that metabolizes xylose using an alternative metabolic pathway, was investigated under aerobic and oxygen-limited culture conditions. Under both culture conditions, FPL-YS30 (xyl3-Ä1) produced a negligible amount of ethanol and converted xylose mainly into xylitol with comparable yields (0.30 and 0.27 g xylitol/g xylose). However, xylose consumption increased five-fold under aerobic compared to oxygen-limited conditions. This suggests that the efficiency of the alternative route of xylose assimilation is affected by respiration. As a result, the FPL-YS30 strain produced 26 g/l of xylitol, and exhibited a higher volumetric productivity (0.22 g xylitol l−1 h−1) under aerobic conditions.

Keywords

Fungi, cultures, culture media, genetic engineering, biotechnology, alcohol, xylitol, Pichia stipitis, xylose, D-xylulokinase

Citation

Jin, Yong-Su; Cruz, Jose; Jeffries, Thomas W. 2005. Xylitol production by a Pichia stipitis D-xylulokinase mutant. Applied microbiology and biotechnology. Vol. 68, no. 1 (2005): pages 42-45.