Download (PDF 132 KB): https://research.fs.usda.gov/download/treesearch/22135.pdf
Abstract
D-Glucose metabolism is thought to be important during wood decay by fungi, not only for anabolic and catabolic purposes of central metabolism, but also as a potential source of peroxide required by extracellular peroxidases. There has been some confusion in the literature as to whether this peroxide-generating activity is of the glucose 1-oxidase or pyranose 2-oxidase (glucose 2-oxidase) type with various fungi or even within the same fungal species. Definitive classification requires accurate identification of the enzymatic products D-glucono- 1,5-lactone and D- arabino-2-hexosulose (glucosone) with glucose 1-oxidase and pyranose 2- oxidase, respectively. We used 13C-NMR to distinguish these reactions starting with 13C-labeled glucose. The use of labeled substrates simplifies analysis and greatly increases detection sensitivity without requiring the isolation or derivatization of metabolites. We synthesized 13C-1-glucosone to study subsequent metabolism with crude enzyme preparations. Preliminary results with Phanerochaete chrysosporium are presented.
Keywords
Phanerochaete chrysosporium,
glucose 1-oxidase,
pyranose 2-oxidase,
D-glucono-1,
5-lactone,
glucosone,
pyranosone dehydratase,
wood-decaying fungi,
peroxidase,
microbial metabolism,
microbial metabolites,
carbon,
fungal physiology,
nuclear magnetic resonance,
enzymes,
Phanerochaete chrysosporium
Citation
de Koker, Theodorus H.; Mozuch, Michael D.; Kersten, Philip J. 2003. Analysis of D-glucose metabolism of wood decay fungi using 13C-NMR and 13C-labeled substrates. Thirty-fourth annual meeting of the International Research Group on Wood Preservation : 2003 May 18-23, Brisbane, Queensland, Australia. Stockholm, Sweden : IRG Secretariat, 2003: 9 pages.
