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Abstract
The role of tetrapartite associations among
Frankia,
Gigaspora margarita (an arbuscular mycorrhizal fungus),
Pseudomonas putida (rhizobacterium), and
Alnus sieboldiana in growth, nitrogen fixation, and mineral acquisition of
A. sieboldiana was investigated. Seedlings of
A. sieboldiana were inoculated with
Frankia isolated from root nodules of alder, followed by inoculation of
G. margarita and
P. putida, and were grown for 5 months in a greenhouse. The seedlings inoculated with
Frankia and
G. margarita together produced the highest biomass of shoots and root nodules. Nitrogen-fixation activity, measured by acetylene reduction assay, was observed when
Frankia was inoculated. The activity, on a per-nodule gram basis, decreased after
G. margarita inoculation, but on a per-plant basis there was no significant difference in the activity among inoculation treatments. The mineral content in the seedlings changed after inoculation with
Frankia, but not after inoculation with
P. putida and/or
G. margarita. The results showed a synergistic interaction among
Frankia, the mycorrhizal fungus, and the rhizobacterium on the growth of
A. sieboldiana.
Citation
Yamanaka, Takashi; Akama, Akio; Li, Ching-Yan; Okabe, Hiroaki. 2005. Growth, nitrogen fixation and mineral acquisition of
Alnus sieboldiana after inoculation of
Frankia together with
Gigaspora margarita and
Pseudomonas putida. Journal of Forest Research. 10: 21-26
