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Abstract
Heterobasidion annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion annosum TC 32-1 was cocultivated at pH 5.6 and 20 1C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
Keywords
Agrobacterium tumefaciens,
Heterobasidion annosum,
green fluorescent protein (GFP),
transformation,
genetic markers,
mycelium,
phytopathogenic fungi,
biological control,
butt rots,
pathogenic fungi,
root rots,
Basidiomycetes,
genetic transformation,
resistance to decay,
decay fungi
Citation
Samils, Nicklas; Elfstrand, Malin; Czederpiltz, Daniel L. Lindner; Fahleson, Jan; Olson, Ake; Dixelius, Christina; Stenlid, Jan. 2006. Development of a rapid and simple Agrobacterium tumefaciens mediated transformation system for the fungal pathogen Heterobasidion annosum. FEMS microbiology letters. Vol. 255 (2006): pages 82-88.
