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Abstract
We describe the structure, organization, and transcriptional impact of repetitive elements within the lignin-degrading basidiomycete, Phanerochaete chrysosporium. Searches of the P. chrysosporium genome revealed five copies of pce1, a 1,750-nt non-autonomous, class II element. Alleles encoding a putative glucosyltransferase and a cytochrome P450 harbor pce insertions and produce incomplete transcripts. Class I elements included pcret1, an intact 8.14-kb gypsy-like retrotransposon inserted within a member of the multicopper oxidase gene family. Additionally, we describe a complex insertion of nested transposons within another putative cytochrome P450 gene. The disrupted allele lies within a cluster of >14 genes, all of which encode family 64 cytochrome P450s. Components of the insertion include a disjoint copia-like element, pcret3, the pol domain of a second retroelement, pcret2, and a duplication of an extended ORF of unknown function. As in the case of the pce elements, pcret1 and pcret2/3 insertions are confined to single alleles, transcripts of which are truncated. The corresponding wild-type alleles are apparently unaVected. In aggregate, P. chrysosporium harbors a complex array of repetitive elements, at least Wve of which directly inXuence expression of genes within families of structurally related sequences.
Keywords
Phanerochaete chrysosporium,
retrotransposons,
multicopper oxidase,
cytochrome P450,
fungi,
genetics,
industrial applications,
molecular genetics,
gene expression,
copper,
lignin,
biodegradation,
genetic transcription,
Basidiomycetes,
oxidases,
wood-decaying fungi,
transposons,
white rot
Citation
Larrondo, Luis F.; Canessa, Paulo; Vicuna, Rafael; Stewart, Philip; Vanden Wymelenberg, Amber; Cullen, Dan. 2007. Structure and transcriptional impact of divergent repetitive elements inserted within Phanerochaete chrysosporium strain RP-78 genes. Molecular genetics and genomics. Vol. 277 (2007): pages 43-55.
