Starch gel electrophoresis of conifer seeds: a laboratory manual

M. Thompson Conkle; Paul D. Hodgskiss; Lucy B. Nunnally; Serena C. Hunter

doi:10.2737/PSW-GTR-64

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Starch gel electrophoresis of conifer seeds: a laboratory manual

Informally Refereed

Authors:

M. Thompson Conkle, Paul D. Hodgskiss, Lucy B. Nunnally, Serena C. Hunter

Year:

1982

Type:

General Technical Report

Station:

Pacific Southwest Research Station

DOI:

https://doi.org/10.2737/PSW-GTR-64

Source:

Gen. Tech. Rep. PSW-GTR-64. Berkeley, CA: Pacific Southwest Forest and Range Experiment Station, Forest Service, U.S. Department of Agriculture: 18 p

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Download (PDF 3.93 MB): https://research.fs.usda.gov/download/treesearch/27487.pdf

Abstract

This manual describes fast, low-cost biochemical procedures for separating enzymes representing numerous genes of forest trees. During electrophoresis the mixture of enzymes from a megagametophyte or embryo of a germinated seed separates in a gel. Specific stains applied to gel slices locate each enzyme. These procedures expand on those developed for crops research. They provide a means for forest geneticists to get urgently needed information on the amount and geographic distribution of genetic variation in conifers for evaluating species relationships, for protecting rare natural populations, and for deciding on breeding programs for commercial species. Electrophoresis of conifers is an alternative to long-term, high-cost field trials.

Keywords

technique, gametophytes, embryos, allozymes, eiectrophoresis

Citation

Conkle, M. Thompson; Hodgskiss, Paul D.; Nunnally, Lucy B.; Hunter, Serena C. 1982. Starch gel electrophoresis of conifer seeds: a laboratory manual. Gen. Tech. Rep. PSW-GTR-64. Berkeley, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Forest and Range Experiment Station. 18 p.