Microsatellites for Lindera species

Craig Echt; D. Deemer; T.L. Kubisiak; C.D. Nelson

Treesearch

Microsatellites for Lindera species

Informally Refereed

Authors:

Craig Echt, D. Deemer, T.L. Kubisiak, C.D. Nelson

Year:

2006

Type:

Scientific Journal

Station:

Southern Research Station

Source:

Molecular Ecology Notes, 6: 1171-1173.

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Abstract

Microsatellite markers were developed for conservation genetic studies of Lindera melissifolia (pondberry), a federally endangered shrub of southern bottomland ecosystems. Microsatellite sequences were obtained from DNA libraries that were enriched for the (AC)_n simple sequence repeat motif. From 35 clone sequences, 20 primer pairs were designed and evaluated. Eleven primer pairs amplified polymorphic marker loci in pondberry while two did so in Lindera benzoin (spicebush). In 46 samples from a single pondberry site the number of microsatellite alleles ranged from two to 11 per locus with observed heterozygosity values of 0.07–0.91.

Keywords

endangered species, pondberry, spicebush

Citation

Echt, Craig S.; Deemer, D.; Kubisiak, T.L.; Nelson, C.D. 2006. Microsatellites for Lindera species. Molecular Ecology Notes, 6: 1171-1173.