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Abstract
Orientation of adjacent genes has been reported to affect their expression in eukaryotic systems, and metabolic engineering also often makes repeated use of a few promoters to obtain high expression. To improve transcriptional control in heterologous expression, we examined how these factors affect gene expression and enzymatic activity in Saccharomyces cerevisiae. We assembled D-xylose reductase (XYL1)and D-xylitol dehydrogenase (XYL2) in four ways. Each pair of genes was placed in two different tandem (1 > 2> or
Keywords
Yeast,
metabolic engineering,
promoter saturation,
gene orientation,
Saccharomyces cerevisiae,
gene expression,
enzyme activities,
genetic transcription,
yeast fungi,
molecular genetics,
growth regulators,
genetic engineering,
microbial metabolism,
regulation,
industrial applications,
wood-decaying fungi,
biotechnology,
enzymes,
plasmids,
xylitol dehydrogenase,
gene promoters,
D-xylose,
decay fungi,
xylose reductase
Citation
Bae, Ju Yun; Laplaza, Jose; Jeffries, Thomas W. 2008. Effects of gene orientation and use of multiple promoters on the expression of XYL1 and XYL2 in Saccharomyces cerevisiae. Applied biochemistry and biotechnology. Vol. 145, no. 3 (2008): Pages 69-78.
