Analyzing genetic diversity in conifers...isozyme resolution by starch gel electrophoresis

M. Thompson Conkle

Treesearch

Analyzing genetic diversity in conifers...isozyme resolution by starch gel electrophoresis

Informally Refereed

Authors:

M. Thompson Conkle

Year:

1972

Type:

Research Note

Station:

Pacific Southwest Research Station

Source:

Res. Note PSW-RN-264. Berkeley, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest and Range Experiment Station. 6 p

Download (PDF 991 KB)

Download (PDF 991 KB): https://research.fs.usda.gov/download/treesearch/32834.pdf

Abstract

Enzymes in forest tree materials can be resolved by starch gel electrophoresis. A gel slab is prepared in a mold assembled from glass and plastic. Wicks containing an aqueous extract of macerated plant material are inserted in the gel and processed. The gel is sliced, stained, examined, and photographed. Isozyme bands produced by differential migration of enzymes indicate genetic segregation and recombination. This technique is familiar in other uses, but the procedure described here is specifically adapted to conifers.

Keywords

coniferae, natural variation, genetic diversity, chemical composition, enzyme

Citation

Conkle, M. Thompson. 1972. Analyzing genetic diversity in conifers...isozyme resolution by starch gel electrophoresis. Res. Note PSW-RN-264. Berkeley, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest and Range Experiment Station. 6 p