Allozymes of linked loci segregate normally in seeds of an Austrian X Japanese red pine hybrid

Abstract

Meiosis in an F₁; hybrid. of Pinus nigra and P. densiflora, which was polymorphic for seven enzyme loci, was examined. To test recombination, lO8 seeds were analyzed by using starch qel electrophoresis of extracts from the haploid tissue of female gametophytes. Three loci segregated independently: acid phosphatase 1, qlutamic oxaloacetic transaminase 3, and qlucose-6-phosphate dehydroqenase 1/2. The four linked loci were alcohol debydroqenase 1, alcohol dehydroqenase 2, glucose-6-phosphate denydroqenase 2, and leucine aminopeptidase 2, arranqed in that order. The recombination percentages between linked loci were 9, 12, and 14, respectively. The order and recombination percentages of the linked loci were similar to values reported for P. sylvestris, another pine in the subsection Sylvestres, as well as for species in other subsections of the qenus. The hybrid provides a means of enriching qenetic variability by producing allelic combinations that do not exist in natural populations.

Citation

Conkle, M. Thompson; Tobolskii, James J. 1981. Allozymes of linked loci segregate normally in seeds of an Austrian X Japanese red pine hybrid. In: Proceedings of the second north central tree improvement conference; 1981 August 5-7; Lincoln, NE. Madison, WI: University of Wisconsin, Department of Forestry: 8 p