Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae)

C. S. Davis; K. E. Mock; Barbara J. Bentz; S. M. Bromilow; N. V. Bartell; B. W. Murray; A. D. Roe; J. E. K. Cooke

doi:10.1111/j.1755-0998.2009.02579.x

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Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae)

Formally Refereed

Authors:

C. S. Davis, K. E. Mock, Barbara J. Bentz, S. M. Bromilow, N. V. Bartell, B. W. Murray, A. D. Roe, J. E. K. Cooke

Year:

2009

Type:

Scientific Journal

Station:

Rocky Mountain Research Station

DOI:

https://doi.org/10.1111/j.1755-0998.2009.02579.x

Source:

2009. Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae). Molecular Ecology Resources. 9(3): 1071-1073.

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Abstract

We isolated 16 polymorphic microsatellite loci in the mountain pine beetle (Dendroctonus ponderosae Hopkins) and developed conditions for amplifying these markers in four multiplex reactions. Three to 14 alleles were detected per locus across two sampled populations. Observed and expected heterozygosities ranged from 0.000 to 0.902 and from 0.100 to 0.830, respectively. Three loci deviated from Hardy-Weinberg equilibrium in one sampled population. One of these loci may be sex linked. These markers will be useful in the study of population structure in this important pest species.

Keywords

Dendroctonus ponderosae, microsatellite, mountain pine beetle

Citation

Davis, C. S.; Mock, K. E.; Bentz, B. J.; Bromilow, S. M.; Bartell, N. V.; Murray, B. W.; Roe, A. D.; Cooke, J. E. K. 2009. Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae). Molecular Ecology Resources. 9(3): 1071-1073.