Estimation of population structure in coastal Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco var. menziesii] using allozyme and microsatellite markers

Konstantin V. Krutovsky; Brad St. Clair; Robert Saich; Valerie Hipkins; David B. Neale

doi:10.1007/s11295-009-0216-y

Treesearch

Estimation of population structure in coastal Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco var. menziesii] using allozyme and microsatellite markers

Informally Refereed

Authors:

Konstantin V. Krutovsky, Brad St. Clair, Robert Saich, Valerie Hipkins, David B. Neale

Year:

2009

Type:

Scientific Journal

Station:

Pacific Northwest Research Station

DOI:

https://doi.org/10.1007/s11295-009-0216-y

Source:

Tree Genetics & Genomes. 5: 641-658

Download (PDF 4.75 MB)

Download (PDF 4.75 MB): https://research.fs.usda.gov/download/treesearch/34937.pdf

Abstract

Characterizing population structure using neutral markers is an important first step in association genetic studies in order to avoid false associations between phenotypes and genotypes that may arise from nonselective demographic factors. Population structure was studied in a wide sample of approximately 1,300 coastal Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco var. menziesii] trees from Washington and Oregon. This sample is being used for association mapping between cold hardiness and phenology phenotypes and single-nucleotide polymorphisms in adaptive-trait candidate genes. All trees were genotyped for 25 allozyme and six simple sequence repeat (SSR) markers using individual megagametophytes. Population structure analysis was done separately for allozyme and SSR markers, as well as for both datasets combined. The parameter of genetic differentiation was standardized to take into account high within-population variation in the SSR loci and to allow comparison with allozyme loci.

Keywords

Allozyme, genetic markers, microsatellites, population structure, Pseudotsuga menziesii, simple sequence repeats (SSR)

Citation

Krutovsky, Konstantin V.; St. Clair, John Bradley; Saich, Robert; Hipkins, Valerie D.; Neale, David B. 2009. Estimation of population structure in coastal Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco var. menziesii] using allozyme and microsatellite markers. Tree Genetics & Genomes. 5: 641-658.