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Abstract
The goal of this work was to develop improved tools for the detection of
Phytophthora ramorum and
P. kernoviae for field and the laboratory use. ImmunoStrip® and ELISA were selected as the test formats for development. Presently, the diagnosis of sudden oak death (SOD) in the national survey of
P. ramorum depends on the use of ELISA to pre-screen samples, and then confirms results with PCR and morphological identification. This approach has some disadvantages because the ELISA has a wide spectrum reaction with
Phytophthora spp. and cross-reacts with
Pythium spp. A faster and more specific serological test to detect
P. ramorum would be very useful in the survey of this pathogen.
P. kernoviae was first detected in rhododendron in Cornwall in 2003 during surveys for
P. ramorum, and the pathogen has been found in New Zealand.
P. kernoviae has not been reported in the U.S. however.
Parent Publication
Citation
Avila, Francisco J.; Schoedel, Barbara; Abad, Z. Gloria; Coffey, Michael D.; Blomquist, Cheryl. 2009. ELISA and ImmunoStrip® for detection of
Phytophthora ramorum,
P. kernoviae, and other
Phytophthora species. In: Frankel, Susan J.; Kliejunas, John T.; Palmieri, Katharine M. 2010. Proceedings of the Sudden Oak Death Fourth Science Symposium. Gen. Tech. Rep. PSW-GTR-229. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station. pp. 95-96
