Agrobacterium-medicated transformation of mature Prunus serotina (black cherry) and regeneration of trangenic shoots

Xiaomei Liu; Paula M. M. Pijut

doi:10.1007/s11240-009-9662-3

Treesearch

Agrobacterium-medicated transformation of mature Prunus serotina (black cherry) and regeneration of trangenic shoots

Informally Refereed

Authors:

Xiaomei Liu, Paula M. M. Pijut

Year:

2010

Type:

Scientific Journal

Station:

Northern Research Station

DOI:

https://doi.org/10.1007/s11240-009-9662-3

Source:

Plant Cell Tissue and Organ Culture. 101: 49-57.

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Abstract

A protocol for Agrobacterium-mediated transformation was developed for in vitro leaf explants of an elite, mature Prunus serotina tree. Agrobacterium tumefaciens strain EHA105 harboring an RNAi plasmid with the black cherry AGAMOUS (AG) gene was used. Bacteria were induced for 12 h with 200 µM acetosyringone for vir gene induction before leaf explant inoculation.

Keywords

Agamous, genetic engineering, leaf explants, RNAi

Citation

Liu, Xiaomei; Pijut, Paula. 2010. Agrobacterium-medicated transformation of mature Prunus serotina (black cherry) and regeneration of trangenic shoots. Plant Cell Tissue and Organ Culture. 101: 49-57. DOI 10.1007/s11240-009-9662-3.