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Abstract
Efficient fermentation of hemicellulosic sugars is critical for the bioconversion of lignocellulosics to ethanol. Efficient sugar uptake through the heterologous expression of yeast and fungal xylose/glucose transporters can improve fermentation if other metabolic steps are not rate limiting. Rectification of cofactor imbalances through heterologous expression of fungal xylose isomerase or modification of cofactor requirements in the yeast oxidoreductase pathway can reduce xylitol production while increasing ethanol yields, but these changes often occur at the expense of xylose utilization rates. Genetic engineering and evolutionary adaptation to increase glycolytic flux coupled with transcriptomic and proteomic studies have identified targets for further modification, as have genomic and metabolic engineering studies in native xylose fermenting yeasts
Keywords
Alcohol,
yeast fungi,
biotechnology,
fungi,
industrial applications,
genetic engineering,
microbial metabolism,
genetics,
lignocellulose,
fermentation,
gene expression,
genomes,
wood-decaying fungi,
genetic transcription,
Saccharomyces cerevisiae,
hemicellulose,
sugars,
glucose,
oxidoreductase,
ethanol,
decay fungi,
xylose,
bioconversion
Citation
Van Vleet, Jennifer; Jeffries, Thomas W. 2009. Yeast metabolic engineering for hemicellulosic ethanol production. Current opinion in biotechnology. Vol. 20, no. 3 (June 2009): pages 300-306.
