Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell culture

Jim Slavicek; Nancy L. Hayes-Plazolles; Mary Ellen Kelly

Treesearch

Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell culture

Informally Refereed

Authors:

Jim Slavicek, Nancy L. Hayes-Plazolles, Mary Ellen Kelly

Year:

1995

Type:

Scientific Journal

Station:

Northern Research Station

Source:

Biological Control. 5: 251-261.

Download (PDF 1.20 MB)

Download (PDF 1.20 MB): https://research.fs.usda.gov/download/treesearch/35988.pdf

Abstract

Four genotypic variants of Lymantria dispar multimucleocapsid nuclear polyhedrosis virus (LdMNPV) were used to investigate the generation of few polyhedra (FP) mutants during viral propagation in the L. dispar 652Y cell line. Titers of budded virus, the percentage of infected cells producting polyhedra, the amount of polyhedra produced per cell, the proportion of virus exhibiting an FP phenotype, virion occlusion characteristics, and changes in genomic DNA digestion profiles were investigated through five serial passages.

Keywords

Lymantria dispar, Lymantria dispar, spongy moth, cell culture, few polyhedra mutants, biocontrol, multinucleocapsid nuclear polyhedrosis virus

Citation

Slavicek, James M.; Hayes-Plazolles, Nancy; Kelly, Mary Ellen. 1995. Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell culture. Biological Control. 5: 251-261.