Identification of a Lymantria dispar nucleopolyhedrovirus isolate that does not accumulate few-polyhedra mutants during extended serial passage in cell culture

Jim Slavicek; Nancy L. Hayes-Plazolles; Mary Ellen Kelly

doi:10.1006/bcon.2001.0969

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Identification of a Lymantria dispar nucleopolyhedrovirus isolate that does not accumulate few-polyhedra mutants during extended serial passage in cell culture

Informally Refereed

Authors:

Jim Slavicek, Nancy L. Hayes-Plazolles, Mary Ellen Kelly

Year:

2001

Type:

Scientific Journal

Station:

Northern Research Station

DOI:

https://doi.org/10.1006/bcon.2001.0969

Source:

Biological Control. 22: 159-168.

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Abstract

During Lymantria dispar multinucleocapsid nucleopolyhedrovirus (LdMNPV) replication in cell culture, few-polyhedra (FP) mutants arise at a high frequency and become predominant after only a few serial passages. The formation of LdMNPV FP mutants was an impediment to successful production of polyhedra in cell culture bioreactors. We have isolated and characterized a strain of LdMNPV, termed 122b, that did not accumulate FP mutants after extended serial passage in cell culture. Wild-type virus strains exhibited decreased polyhedra production and increased budded virus (BV) release, and FP mutants were the predominant virus type present after only a few serial passages in L. dispar 652Y cells. In contrast, isolate 122b exhibited stable polyhedra and BV production through 14 serial passages.

Keywords

baculovirus, Lymantria dispar nucleopolyhedrovirus, few-polyhedra mutant, cell culture, stable polyhedra production, Lymantria dispar, biological control

Citation

Slavicek, James M.; Hayes-Plazolles, Nancy; Kelly, Mary Ellen. 2001. Identification of a Lymantria dispar nucleopolyhedrovirus isolate that does not accumulate few-polyhedra mutants during extended serial passage in cell culture. Biological Control. 22: 159-168.