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Abstract
This study demonstrated Sulfite Pretreatment to Overcome Recalcitrance of Lignocellulose (SPORL) for robust conversion of softwood through enzymatic hydrolysis. At a sodium bisulfite charge around 9%, over 90% cellulose conversion could be achieved when spruce wood chips were pretreated at 180°C with pH near 2. For lodgepole pine, pretreatment liquor initial pH had no effect on enzymatic cellulose conversion in the tested range of 1.9– 4.2 with a sodium bisulfite charge of 8%. The dissolution of glucan during pretreatment was only about 10%. Most of the hemicelluloses were recovered as monomeric fermentable sugars in the pretreatment spent liquor. SPORL was developed based on sulfite pulping, and therefore has a precedent for excellent scalability for future commercialization.
Keywords
Alcohol,
fuelwood,
carbohydrates,
spruce,
biomass energy,
biomass utilization,
cellulose,
hemicellulose,
hydrolysis,
wood chips,
pretreatment,
feedstock,
enzymes,
industrial applications,
biotechnology,
sulfuric acid,
biotechnology,
lodgepole pine,
fermentation,
lignocellulose,
sugars,
glucose,
ethanol,
SPORL,
bioconversion,
biorefining,
saccharification,
sodium bisulfite,
chips,
lodgepole pine,
wood extractives,
chemical utilization,
sulfonation,
sulfite liquors
Citation
Zhu, J.Y.; Pan, X.J.; Zhu, W.; Wang, G.S.; Gleisner, R. 2009. Robust and efficient enzymatic saccharification of softwoods by SPORL. In: Proceedings of the 15th International Symposium on Wood, Fiber and Pulping Chemistry, 2009 June 15-18, Oslo, Norway. (P-033) Oslo, Norway : Congress-Conference AS, 2009: [3] p.
