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Abstract
In scale-up, the potential of ethanol production by dilute sulfuric acid pretreatment using corncob was investigated. Pretreatments were performed at 170 °C with various acid concentrations ranging from 0% to 1.656% based on oven dry weight. Following pretreatment, pretreated biomass yield ranged from 59% to 67%. More than 90% of xylan was removed at 0.828% of sulfuric acid. At same pretreatment condition, the highest glucose yield obtained from pretreated biomass by enzymatic hydrolysis was about 76%, based on a glucan content of 37/100 g. In hydrolysate obtained by pretreatment, glucose concentration was low, while xylose concentration was significantly increased above 0.368% of sulfuric acid. At 1.656% of sulfuric acid, xylose and glucose concentration was highest. In subsequent, fermentation with hydrolysate, maximal ethanol yield was attained after 24 h with 0.368% of sulfuric acid. The fermentation efficiency of hydrolysate obtained by enzymatic hydrolysis reached a maximum of 75% at an acid charge of 0.368%.
Keywords
Pretreatment,
enzymatic hydrolysis,
corncobs,
ethanol,
fermentation,
corn residues,
biomass utilization,
biomass energy,
fungi,
biotechnology,
yeast fungi,
enzymes,
industrial applications,
hydrolysis,
Pichia stipitis,
glucose,
chemical reactions,
sugars,
sulfuric acid,
feedstock,
alcohol,
biomass fuel,
biorefining,
bioconversion,
corn as fuel,
xylan,
decay fungi,
acid hydrolysis,
xylose,
hydrolysates
Citation
Lee, Jae-Won; Zhu, J.Y.; Scordia, Danilo; Jeffries, Thomas W. 2011. Evaluation of ethanol production from corncob using Scheffersomyces (Pichia) stipitis CBS 6054 by volumetric scale-up. Applied Biochemistry and Biotechnology. 165(3/4): 814-822.
