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Abstract
Enzymatic hydrolysis of lignocellulosic biomass in a high shear environment was examined. The conversion of cellulose to glucose in samples mixed in a torque rheometer producing shear flows similar to those found in twin screw extruders was greater than that of unmixed samples. In addition, there is a synergistic effect of mixing and enzymatic hydrolysis; mixing increases the rate of cellulose conversion while the increased conversion facilitates mixing. The synergy appears to result in part from particle size reduction, which is more significant when hydrolysis occurs during intense mixing.
Keywords
Suspensions,
pulping,
cellulose fibers,
biomass energy,
biotechnology,
feedstock,
glucose,
lignocellulose,
biodegradation,
hydrolysis,
enzymes,
industrial applications,
cellulose,
ethanol,
biomass,
cellulase,
corn stover,
corn stover as fuel,
corn residues,
chemical reactions,
rheometers,
shear,
rheology,
cotton,
crop residues,
agricultural wastes,
lignocellulosic biomass,
enzymatic hydrolysis,
mixing,
viscosity,
fibers,
plant fibers,
biomass fuel,
bioconversion,
biorefining,
particle size,
corn as fuel,
alcohol,
acid hydrolysis
Citation
Samaniuk, Joseph R.; Scott, C. Tim; Root, Thatcher W.; Klingenberg, Daniel J. 2011. The effect of high intensity mixing on the enzymatic hydrolysis of concentrated cellulose fiber suspensions. Bioresource technology. Vol. 102, no. 6 (Mar. 2011): p. 4489-4494.