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Abstract
Building on our laboratory-scale optimization, oxalic acid was used to pretreat corncobs on the pilotscale. The hydrolysate obtained after washing the pretreated biomass contained 32.55 g/l of xylose, 2.74 g/l of glucose and low concentrations of inhibitors. Ethanol production, using Scheffersomyces stipitis, from this hydrolysate was 10.3 g/l, which approached the predicted value of 11.9 g/l. Diafiltration using a membrane system effectively reduced acetic acid in the hydrolysate, which increased the fermentation rate. The hemicellulose content of the recovered solids decreased from 27.86% before pretreatment to only 6.76% after pretreatment. Most of the cellulose remained in the pretreated biomass. The highest ethanol production after simultaneous saccharification and fermentation (SSF) of washed biomass with S. stipitis was 21.1 g/l.
Keywords
Lignocellulose,
biodegradation,
cellulose,
biotechnology,
lignin,
biomass energy,
corncobs,
biomass,
ethanol,
sugars,
pretreatment,
chemical reactions,
fermentation,
oxalic acid,
hemicellulose,
Pichia stipitis,
fungi,
yeast fungi,
glucose,
acetic acid,
decay fungi,
corn as fuel,
biomass fuel,
bioconversion,
biorefining,
alcohol,
biofuels,
saccharification,
hydrolysates,
xylose,
scale-up
Citation
Lee, Jae-Won; Houtman, Carl J.; Kim, Hye-Yun; Choi, In-Gyu; Jeffries, Thomas W. 2011. Scale-up study of oxalic acid pretreatment of agricultural lignocellulosic biomass for the production of bioethanol. Bioresource technology. Vol. 102, no. 16 (Aug. 2011): p. 7451-7456.
