Cytogenetic Analysis of Populus trichocarpa - Ribosomal DNA, Telomere Repeat Sequence, and Marker-selected BACs

Nurul Islam-Faridi; C. Dana Nelson; S.P. DiFazio; L.E. Gunter; G.A. Tuskan

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Cytogenetic Analysis of Populus trichocarpa - Ribosomal DNA, Telomere Repeat Sequence, and Marker-selected BACs

Formally Refereed

Authors:

Nurul Islam-Faridi, C. Dana Nelson, S.P. DiFazio, L.E. Gunter, G.A. Tuskan

Year:

2009

Type:

Scientific Journal

Station:

Southern Research Station

Source:

Cytogenet Genome Res 125:74-80

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Abstract

The 185-285 rDNA and 55 rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 185-285 rDNA sites and one 55 rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.

Keywords

BAC, FISH, Populus trichocarpa, rDNA, Telomere

Citation

lslam-Faridia, M.N.; Nelson, C.D.; DiFazio, S.P.; Gunter, L.E.; Tuskan, G.A. 2009. Cytogenetic Analysis of Populus trichocarpa - Ribosomal DNA, Telomere Repeat Sequence, and Marker-selected BACs. Cytogenet Genome Res 125:74-80.