A sex-averaged genetic linkage map in coastal Douglas-fir (Pseudotsuga menziesii [Mirb] Franco var menziesii) based on RFLP and RAPD markers

Kathie Jermstad; D.L. Bassoni; N.C. Wheeler; D.B. Neale

doi:10.1007/s001220050953

Treesearch

A sex-averaged genetic linkage map in coastal Douglas-fir (Pseudotsuga menziesii [Mirb] Franco var menziesii) based on RFLP and RAPD markers

Formally Refereed

Authors:

Kathie Jermstad, D.L. Bassoni, N.C. Wheeler, D.B. Neale

Year:

1998

Type:

Scientific Journal

Station:

Pacific Southwest Research Station

DOI:

https://doi.org/10.1007/s001220050953

Source:

Theor Appl Genet 97:762-770

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Abstract

We have constructed a sex-averaged genetic linkage map in coastal Douglas-fir ( Pseudotsuga menziesii [Mirb.] Franco var menziesii) using a three-generation outcrossed pedigree and molecular markers. Our research objectives are to learn about genome organization and to identify markers associated with adaptive traits. The map reported here is comprised of 141 markers organized into 17 linkage groups and covers 1,062 centiMorgans (cM). Of the markers positioned on the map, 94 were derived from a Douglas-fir complimentary-DNA (cDNA) library that was constructed from new-growth needle tissue. Other markers include 11 Douglas-fir genomic-DNAs, 20 loblolly pine (Pinus taeda L.) cDNAs, 15 random amplifed polymorphic DNAs (RAPDs) and a PCR amplifed phytochrome probe. A high degree of variation was detected in each of the two parents of our mapping population, and many of the restriction fragment length polymorphism (RFLP) and RAPD phenotypes were complex. Marker data were analyzed for linkage using mapping software JOINMAP version 2.0.

Keywords

RFLP, RAPD, Genetic map, Null loci, Gene family

Citation

Jermstad, K.D.; Bassoni, D.L.; Wheeler, N.C.; Neale, D.B. 1998. A sex-averaged genetic linkage map in coastal Douglas-fir (Pseudotsuga menziesii [Mirb] Franco var menziesii) based on RFLP and RAPD markers. Theor Appl Genet 97:762-770.