Genetic fingerprinting of longleaf pine seed orchard clones following Hurricane Hugo

Kathie Jermstad; P.A. Guge; E.R. Carroll; S.T. Friedman; D.B. Neale

Treesearch

Genetic fingerprinting of longleaf pine seed orchard clones following Hurricane Hugo

Formally Refereed

Authors:

Kathie Jermstad, P.A. Guge, E.R. Carroll, S.T. Friedman, D.B. Neale

Year:

1993

Type:

Scientific Journal

Station:

Pacific Southwest Research Station

Source:

Tree Planter's Notes. 44(4): 157-160

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Abstract

Isozyme and restriction fragment length polymorphism (RFLP) markers were used to determine the genetic identities of 12 longleaf pine (Pinus palustrus Mill.) ramets whose identities came into question after Hurricane Hugo. Isozyme assays were performed for 12 enzyme systems representing 15 loci. Variation at 6 loci revealed unique identities for 6 ramets. Four RFLP probes showed that 9 of the 12 ramets were genetically unique. This study confirms the power of isozyme analysis for determining genetic identity of seed orchard clones and, in addition, demonstrates the increased power of genetic discrimination offered by RFLP analysis.

Citation

Jermstad, K. D.; Guge, P.A.; Carroll, E.R.; Friedman, S.T.; Neale, D.B. 1993. Genetic fingerprinting of longleaf pine seed orchard clones following Hurricane Hugo. Tree Planter's Notes. 44(4): 157-160.