Microsatellite loci development in mycoheterotrophic Corallorhiza maculata with amplification in C. mertensiana

Sarah E. Hopkins; D. Lee Taylor

Treesearch

Microsatellite loci development in mycoheterotrophic Corallorhiza maculata with amplification in C. mertensiana

Formally Refereed

Authors:

Sarah E. Hopkins, D. Lee Taylor

Year:

2011

Type:

Scientific Journal

Station:

Pacific Northwest Research Station

Source:

American Journal of Botany. e253-e255. doi:10.3732/ajb.1100061

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Abstract

Microsatellite primers were developed for the first time in the species Corallorhiza maculata, a nonphotosynthetic orchid that is becoming a model for studying mycorrhizal specificity. Eight polymorphic microsatellite markers were developed using an enrichment and cloning protocol. The number of alleles for each locus ranged from two to seven. The loci were tested in three populations, and the resulting high FsT values suggested that these loci have utility in illuminating population structure. Cross-amplification was also achieved in the sister species C. mertensiana. These microsatellite markers will be useful in further study of the population genetics of this species, including that of previously described mycorrhizal races.

Keywords

Corallorhiza maculata, cross-species amplification, microsatellite, orchids

Citation

Hopkins, Sarah E.; Taylor, D. Lee. 2011. Microsatellite loci development in mycoheterotrophic Corallorhiza maculata (ORCHIDACEAE) with amplification in C. mertensiana. American Journal of Botany. e253-e255. doi:10.3732/ajb.1100061.