Methods for assessing Phytophthora ramorum chlamydospore germination

Joyce Eberhart; Elilzabeth Stamm; Jennifer Parke

Treesearch

Methods for assessing Phytophthora ramorum chlamydospore germination

Informally Refereed

Authors:

Joyce Eberhart, Elilzabeth Stamm, Jennifer Parke

Year:

2013

Type:

General Technical Report

Station:

Pacific Southwest Research Station

Source:

In: Frankel, S.J.; Kliejunas, J.T.; Palmieri, K.M.; Alexander, J.M. tech. coords. Proceedings of the sudden oak death fifth science symposium. Gen. Tech. Rep. PSW-GTR-243. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 130-133

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Abstract

Germination of chlamydospores is difficult to accurately assess when chlamydospores are attached to remnants of supporting hyphae. We developed two approaches for closely observing and rigorously quantifying the frequency of chlamydospore germination in vitro. The plate marking and scanning method was useful for quantifying germination of large numbers of chlamydospores over a 7-day period. A method involving time lapse photography of microscope slide cultures was effective for visualizing the process of germination over shorter time periods.

Parent Publication

Proceedings of the sudden oak death fifth science symposium

Keywords

Phytophthora ramorum, chlamydospore, germination, assessment, banana slug

Citation

Eberhart, Joyce; Stamm, Elilzabeth; Parke, Jennifer. 2013. Methods for assessing Phytophthora ramorum chlamydospore germination. In: Frankel, S.J.; Kliejunas, J.T.; Palmieri, K.M.; Alexander, J.M. tech. coords. Proceedings of the sudden oak death fifth science symposium. Gen. Tech. Rep. PSW-GTR-243. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 130-133.