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Abstract
Phytophthora ramorum characteristically produces large amounts of chlamydospores
in vitro, but the role of these propagules in the disease cycle remains unclear. Germination is difficult to observe and quantify if chlamydospores are not free of mycelium, and the low frequency of germination commonly reported suggests that requirements for germination may not have been entirely met. Here, we conducted germination experiments factoring for chlamydospore age, chlamydospore isolation method, and nutrient medium. Chlamydospore germination frequency in each of the treatments ranged from 0 to 70 percent. Results suggest that chlamydospore age, isolation method, and nutrient media significantly affect chlamydospore germination.
Parent Publication
Citation
Shaffer, Justin P.; Parke, Jennifer L. 2013. Germination of
Phytophthora ramorum chlamydospores: a comparison of separation method and chlamydospore age. In: Frankel, S.J.; Kliejunas, J.T.; Palmieri, K.M.; Alexander, J.M. tech. coords. Proceedings of the sudden oak death fifth science symposium. Gen. Tech. Rep. PSW-GTR-243. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: 160-163.
