First results with a lab-on-a-chip system for a fast Phytophthora diagnosis.

Sonja Horatzek; Stephan König; Stefan Wagner; Sabine Werres; Lydia Schwenkbier; Karina Weber; Jörg Weber

Treesearch

First results with a lab-on-a-chip system for a fast Phytophthora diagnosis.

Informally Refereed

Authors:

Sonja Horatzek, Stephan König, Stefan Wagner, Sabine Werres, Lydia Schwenkbier, Karina Weber, Jörg Weber

Year:

2013

Type:

General Technical Report

Station:

Pacific Southwest Research Station

Source:

In: Frankel, S.J.; Kliejunas, J.T.; Palmieri, K.M.; Alexander, J.M. tech. coords. Proceedings of the sudden oak death fifth science symposium. Gen. Tech. Rep. PSW-GTR-243. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: p. 168

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Abstract

For Phytophthora spp. that are quarantine or regulated organisms, highly specific and sensitive diagnostic tools are recommended for surveys and monitoring. Furthermore, these diagnostic techniques should give results within a short time and should be not be too expensive. The techniques currently used for routine diagnosis of Phytophthora spp. in plant tissue are mainly molecular techniques (conventional and real-time PCR) and direct isolation. They require that samples must be brought to a diagnostic lab with specific equipment. This takes time and means financial losses for the commercial nursery industry because they have to stop plant sales until results are available. Furthermore, with PCR, only a single Phytophthora sp. can be detected per run. Therefore, techniques that can be used directly in the field and that can detect multiple Phytophthora spp. at a time would be preferred.

Parent Publication

Proceedings of the sudden oak death fifth science symposium

Keywords

Sudden oak death, Phytophthora ramorum, invasive species, tanoak, Notholithocarpus densiflorus, coast live oak, Quercus agrifolia, Japanese larch, Larix kaempferi

Citation

Horatzek, Sonja; König, Stephan; Wagner, Stefan; Werres, Sabine; Schwenkbier, Lydia; Weber, Karina; Weber, Jörg. 2013. First results with a lab-on-a-chip system for a fast Phytophthora diagnosis. In: Frankel, S.J.; Kliejunas, J.T.; Palmieri, K.M.; Alexander, J.M. tech. coords. Proceedings of the sudden oak death fifth science symposium. Gen. Tech. Rep. PSW-GTR-243. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: p. 168.