Phenotypic selection of a wild Saccharomyces cerevisiae strain for simultaneous saccharification and co-fermentation of AFEX pretreated corn stover

Mingie Jin; Cory Sarks; Christa Gunawan; Benjamin D. Bice; Shane P. Simonett; Ragothaman Avanasi Narasimhan; LAURA BETH WILLIS; Bruce E. Dale; Venkatesh Balan; Trey K. Sato

doi:10.1186/1754-6834-6-108

Treesearch

Phenotypic selection of a wild Saccharomyces cerevisiae strain for simultaneous saccharification and co-fermentation of AFEX pretreated corn stover

Formally Refereed

Authors:

Mingie Jin, Cory Sarks, Christa Gunawan, Benjamin D. Bice, Shane P. Simonett, Ragothaman Avanasi Narasimhan, LAURA BETH WILLIS, Bruce E. Dale, Venkatesh Balan, Trey K. Sato

Year:

2013

Type:

Scientific Journal

Station:

Forest Products Laboratory

DOI:

https://doi.org/10.1186/1754-6834-6-108

Source:

Biotechnology for Biofuels, Volume 6, Number 108, 2013; 14 p.

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Download (PDF 686 KB): https://research.fs.usda.gov/download/treesearch/44399.pdf

Abstract

Simultaneous saccharification and co-fermentation (SSCF) process involves enzymatic hydrolysis of pretreated lignocellulosic biomass and fermentation of glucose and xylose in one bioreactor. The optimal temperatures for enzymatic hydrolysis are higher than the standard fermentation temperature of ethanologenic Saccharomyces cerevisiae. Moreover, degradation products resulting from biomass pretreatment impair fermentation of sugars, especially xylose, and can synergize with high temperature stress. One approach to resolve both concerns is to utilize a strain background with innate tolerance to both elevated temperatures and degradation products.

Keywords

Thermo-tolerance, Xylose fermentation, S cerevisiae, SSCF, AFEX, Degradation products, Ethanol

Citation

Jin, Mingie; Sarks, Cory; Gunawan, Christa; Bice, Benjamin D.; Simonett, Shane P.; Narasimhan, Ragothaman Avanasi; Willis, Laura B.; Dale, Bruce E.; Balan, Venkatesh; Sato, Trey K. 2013. Phenotypic selection of a wild Saccharomyces cerevisiae strain for simultaneous saccharification and co-fermentation of AFEX(TM) pretreated corn stover. Biotechnology for Biofuels. 6(108): 14 p.