High-resolution genetic and physical mapping of eastern filbert blight resistance in hazelnut

Abstract

Eastern filbert blight (EFB), caused by the pyrenomycete Anisogramma anomala, is a serious threat to the hazelnut (Corylus avellana L.) industry in the Pacific Northwest. A dominant allele at a single locus from the obsolete pollenizer 'Gasaway' confers a very high level of resistance, and has been extensively used in the hazelnut breeding program at Oregon State University. Several linked random amplified polymorphic DNA (RAPD) markers have been identified. Map-based cloning of the EFB resistance gene from 'Gasaway' was initiated by constructing a bacterial artificial chromosome (BAC) library for 'Jefferson' which is heterozygous for resistance (Sathuvalli and Mehlenbacher 2011). The BAC library was constructed using the cloning enzyme MboI and the vector pECBAC1 (BamHI site). The library consists of 39,936 clones arrayed in microtiter plates with an average insert size of 117 kb and estimated coverage of 12 genome equivalents.

Parent Publication

• Proceedings of the fourth international workshop on the genetics of host-parasite interactions in forestry: Disease and insect resistance in forest trees

Citation

Sathuvall, Vidyasagar; Mehlenbacher, Shawn A. 2012. High-resolution genetic and physical mapping of eastern filbert blight resistance in hazelnut. In: Sniezko, Richard A.; Yanchuk, Alvin D.; Kliejunas, John T.; Palmieri, Katharine M.; Alexander, Janice M.; Frankel, Susan J., tech. coords. Proceedings of the fourth international workshop on the genetics of host-parasite interactions in forestry: Disease and insect resistance in forest trees. Gen. Tech. Rep. PSW-GTR-240. Albany, CA: Pacific Southwest Research Station, Forest Service, U.S. Department of Agriculture. pp. 174-175.