Characterization of EST-based SSR loci in the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae)

B.M.T. Brunet; D. Doucet; Brian R. R. Sturtevant; F.A.H. Sperling

doi:10.1007/s12686-012-9847-7

Treesearch

Characterization of EST-based SSR loci in the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae)

Formally Refereed

Authors:

B.M.T. Brunet, D. Doucet, Brian R. R. Sturtevant, F.A.H. Sperling

Year:

2013

Type:

Scientific Journal

Station:

Northern Research Station

DOI:

https://doi.org/10.1007/s12686-012-9847-7

Source:

Conservation Genetic Resources. 5: 541-544.

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Abstract

After identifying 114 microsatellite loci from Choristoneura fumiferana expressed sequence tags, 87 loci were assayed in a panel of 11 wild-caught individuals, giving 29 polymorphic loci. Further analysis of 20 of these loci on 31 individuals collected from a single population in northern Minnesota identified 14 in Hardy-Weinberg equilibrium.

Keywords

Spruce budworm, Expressed sequence tag, Microsatellite, Simple sequence repeat, Choristoneura fumiferana, Tortricidae

Citation

Brunet, B.M.T.; Doucet, D.; Sturtevant, B.R.; Sperling, F.A.H. 2013. Characterization of EST-based SSR loci in the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae). Conservation Genetic Resources. 5: 541-544.