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From diagnostics to metagenomics: Applications of DNA-based tools in forest pathology

Informally Refereed
Download (PDF 693 KB): https://research.fs.usda.gov/download/treesearch/45082.pdf

Abstract

Advances in molecular technology provide an accessible set of tools to 1) help forest pathologists detect, identify, and monitor forest pathogens, 2) examine the evolutionary relationships and global distributions of forest pathogens and their hosts, 3) assess the diversity and structure of host and pathogen populations, and 4) evaluate the structure and function of genes, as well as their levels of expression, within species and within communities. This paper briefly outlines DNA-based tools, including molecular diagnostics, phylogenetics, population genetics, genomics, and metagenomics, and discusses how each has been or could be applied in the field of forest pathology.

Citation

Ross-Davis, Amy L.; Kim, Mee-Sook; Stewart, Jane E.; Hanna, John W.; Shaw, John D.; Klopfenstein, Ned B. 2013. From diagnostics to metagenomics: Applications of DNA-based tools in forest pathology. In: Browning, John; Palacios, Patsy, comps. Proceedings of the 60th Annual Western International Forest Disease Work Conference; October 8-12 October 2012; Tahoe City, CA. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Forest Health Technology and Enterprise Team. p. 91-99.