Lignosulfonate-mediated cellulase adsorption: enhanced enzymatic saccharification of lignocellulose through weakening nonproductive binding to lignin

Zhaojiang Wang; Junyong Zhu; Yingjuan Fu; Menghua Qin; Zhiyong Shao; Jungang Jiang; Fang Yang

doi:10.1186/1754-6834-6-156;2013

Treesearch

Lignosulfonate-mediated cellulase adsorption: enhanced enzymatic saccharification of lignocellulose through weakening nonproductive binding to lignin

Formally Refereed

Authors:

Zhaojiang Wang, Junyong Zhu, Yingjuan Fu, Menghua Qin, Zhiyong Shao, Jungang Jiang, Fang Yang

Year:

2013

Type:

Scientific Journal

Station:

Forest Products Laboratory

DOI:

https://doi.org/10.1186/1754-6834-6-156;2013

Source:

Biotechnology for Biofuels Volume 6, 2013; 10 p.

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Download (PDF 3.24 MB): https://research.fs.usda.gov/download/treesearch/45304.pdf

Abstract

Thermochemical pretreatment of lignocellulose is crucial to bioconversion in the fields of biorefinery and biofuels. However, the enzyme inhibitors in pretreatment hydrolysate make solid substrate washing and hydrolysate detoxification indispensable prior to enzymatic hydrolysis. Sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) is a relatively new process, but has demonstrated robust performance for sugar and biofuel production from woody biomass in terms of yield and energy efficiency. This study demonstrated the advantage of SPORL pretreatment whereby the presentation of lignosulfonate (LS) renders the hydrolysate non-inhibitory to cellulase (Cel) due to the formation of lignosulfonate-cellulase complexes (LCCs) which can mediate the Cel adsorption between lignin and cellulose, contrary to the conventional belief that pretreatment hydrolysate inhibits the enzymatic hydrolysis unless detoxified.

Keywords

Enzymatic saccharification/hydrolysis, Lignin/lignosulfonate, Nonproductive/nonspecific binding/adsorption, Polyelectrolyte-protein complexes, Pretreatment

Citation

Wang, Zhaojiang; Zhu, JY; Fu, Yingjuan; Qin, Menghua; Shao, Zhiyong; Jiang, Jungang; Yang, Fang. 2013. Lignosulfonate-mediated cellulase adsorption: enhanced enzymatic saccharification of lignocellulose through weakening nonproductive binding to lignin. Biotechnology for Biofuels Volume 6, 2013; 10 p.