The cloning and characterization of a poplar stomatal density gene

Shaneka Lawson; Paula M. M. Pijut; Charles H. Michler

doi:10.1007/s13258-014-0177-x

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The cloning and characterization of a poplar stomatal density gene

Formally Refereed

Authors:

Shaneka Lawson, Paula M. M. Pijut, Charles H. Michler

Year:

2014

Type:

Scientific Journal

Station:

Northern Research Station

DOI:

https://doi.org/10.1007/s13258-014-0177-x

Source:

Genes & Genomics. 36(4): 427-441.

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Download (PDF 1.64 MB): https://research.fs.usda.gov/download/treesearch/46283.pdf

Abstract

EPIDERMAL PATTERNING FACTOR1 (EPF1) is a well characterized negative regulator of cell division in Arabidopsis thaliana (AtEPF1) where the primary region of localization is the leaf. However, little data have been reported on the role of EPF1 in other plant species. In this study, the EPF1 gene from Arabidopsis and the newly identified poplar ortholog from Populus trichocarpa (PtaEPF1) were overexpressed in a hybrid poplar genotype. We attempted to identify the physiological role of PtaEPF1. Gene overexpression experiments were performed to determine if and how stomatal density (SD) numbers were affected. The poplar 717-1B4 (P. tremula × P. alba) genotype was used in the study. Results presented here suggest that overexpression of Pta

Keywords
stomatal density, tree physiology, poplar, transgenics

Citation
Lawson, Shaneka S.; Pijut, Paula M.; Michler, Charles H. 2014. The cloning and characterization of a poplar stomatal density gene. Genes & Genomics. 36(4): 427-441.

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