Temporal Alterations in the Secretome of the Selective Ligninolytic Fungus Ceriporiopsis subvermispora during growth on Aspen Wood Reveal this Organism's Strategy for Degrading Lignocellulose

Abstract

The white-rot basidiomycetes efficiently degrade all wood cell wall polymers. Generally, these fungi simultaneously degrade cellulose and lignin, but certain organisms, such as Ceriporiopsis subvermispora, selectively remove lignin in advance of cellulose degradation. However, relatively little is known about themechanismof selective ligninolysis. To address this issue, C. subvermispora was grown in liquidmediumcontaining ball-milled aspen, and nano-liquid chromatography-tandemmass spectrometry was used to identify and estimate extracellular protein abundance over time. Several manganese peroxidases and an aryl alcohol oxidase, both associated with lignin degradation, were identified after 3 days of incubation. A glycoside hydrolase (GH) family 51 arabinofuranosidase was also identified after 3 days but then successively decreased in later samples. Several enzymes related to cellulose and xylan degradation, such as GH10 endoxylanase, GH5_5 endoglucanase, and GH7 cellobiohydrolase, were detected after 5 days. Peptides corresponding to potential cellulose-degrading enzymes GH12, GH45, lytic polysaccharide monooxygenase, and cellobiose dehydrogenase weremost abundant after 7 days. This sequential production of enzymes provides a mechanism consistent with selective ligninolysis by C. subvermispora.

Citation

Hori, Chiaki; Gaskell, Jill; Igarashi, Kiyohiko; Kersten, Phil; Mozuch, Michael; Samejima, Masahiro; Cullen, Dan. 2014. Temporal Alterations in the Secretome of the Selective Ligninolytic Fungus Ceriporiopsis subvermispora during growth on Aspen Wood Reveal this Organism's Strategy for Degrading Lignocellulose. Appl. Environ. Microbiol. 2014, Volume 80, Number 7, pp. 2062-2070.