3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography

Michael C. Martin; Charlotte Dabat-Blondeau; Miriam Unger; Julia Sedlmair; Dilworth Y. Parkinson; Hans A. Bechtel; Barbara L. Illman; Jonathan M. Castro; Marco Keiluweit; David Buschke; Brenda Ogle; Michael J. Nasse; Carol J. Hirschmugl

doi:10.1038/nmeth.2596

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3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography

Formally Refereed

Authors:

Michael C. Martin, Charlotte Dabat-Blondeau, Miriam Unger, Julia Sedlmair, Dilworth Y. Parkinson, Hans A. Bechtel, Barbara L. Illman, Jonathan M. Castro, Marco Keiluweit, David Buschke, Brenda Ogle, Michael J. Nasse, Carol J. Hirschmugl

Year:

2013

Type:

Scientific Journal

Station:

Forest Products Laboratory

DOI:

https://doi.org/10.1038/nmeth.2596

Source:

Nature Methods, Volume 10, Number 9, SEPTEMBER 2013 pp. 861-866.

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Abstract

We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).

Keywords

chemical imaging, cell walls, lignocellulose, FTIR, synchrotrons

Citation

Martin, Michael C.; Dabat-Blondeau, Charlotte; Unger, Miriam; Sedlmair, Julia; Parkinson, Dilworth Y.; Bechtel, Hans A.; Illman, Barbara; Castro, Jonathan M.; Keiluweit, Marco; Buschke, David; Ogle, Brenda; Nasse, Michael J.; Hirschmugl, Carol J. 2013. 3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography. Nature Methods, Volume 10, Number 9, SEPTEMBER 2013 pp. 861-866.