Agrobacterium-mediated genetic transformation of Fraxinus americana hypocotyls

Kaitlin J. Palla; Paula M. M. Pijut

doi:10.1007/s11240-014-0630-1

Treesearch

Agrobacterium-mediated genetic transformation of Fraxinus americana hypocotyls

Formally Refereed

Authors:

Kaitlin J. Palla, Paula M. M. Pijut

Year:

2015

Type:

Scientific Journal

Station:

Northern Research Station

DOI:

https://doi.org/10.1007/s11240-014-0630-1

Source:

Plant Cell, Tissue and Organ Culture. 120(2): 631-641.

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Abstract

An Agrobacterium tumefaciens-mediated genetic transformation system was successfully developed for white ash (Fraxinus americana) using hypocotyls as the initial explants. Hypocotyls isolated from mature embryos germinated on Murashige and Skoog (MS) medium supplemented with 22.2 µM 6-benzyladenine (BA) and 0.5 µM thidiazuron (TDZ) were transformed using A. tumefaciens strain EHA105 harboring the binary vector pq35GR containing a fusion gene between neomycin phosphotransferase (nptII) and gusA, as well as an enhanced green fluorescent protein (EGFP).

Keywords

Fraxinus, Genetic transformation, Organogenesis, Regeneration, White ash

Citation

Palla, Kaitlin J.; Pijut, Paula M. 2015. Agrobacterium-mediated genetic transformation of Fraxinus americana hypocotyls. Plant Cell, Tissue and Organ Culture. 120(2): 631-641.