Measuring aerobic respiration in stream ecosystems using the resazurin-resorufin system

R. González-Pinzón; R. Haggerty; D.D. Myrold

doi:10.1029/2012JG001965

Treesearch

Measuring aerobic respiration in stream ecosystems using the resazurin-resorufin system

Formally Refereed

Authors:

R. González-Pinzón, R. Haggerty, D.D. Myrold

Year:

2012

Type:

Scientific Journal

Station:

Pacific Northwest Research Station

DOI:

https://doi.org/10.1029/2012JG001965

Source:

Journal of Geophysical Research

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Abstract

The use of smart tracers to study hydrologic systems is becoming more widespread. Smart tracers are compounds that irreversibly react in the presence of a process or condition under investigation. Resazurin (Raz) is a smart tracer that undergoes an irreversible reduction to resorufin (Rru) in the presence of cellular metabolic activity. We quantified the relationship between the transformation of Raz and aerobic bacterial respiration in pure culture experiments using two obligate aerobes and two facultative anaerobes, and in colonized surface and shallow (

Keywords

aerobic, respiration, stream, ecosystem, hydrologic, resazurin-resorufin

Citation

González-Pinzón, R.; Haggerty, R.; Myrold, D.D. 2012. Measuring aerobic respiration in stream ecosystems using the resazurin-resorufin system. Journal of Geophysical Research. 117(G00N06): 1-10.